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gentle cell dissociation reagent stem cell technologies; 07174  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc gentle cell dissociation reagent stem cell technologies; 07174
    Gentle Cell Dissociation Reagent Stem Cell Technologies; 07174, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gentle+cell+dissociation+reagent+stem+cell/gentle+cell+dissociation+reagent+stem+cell+technologies++07174/pmc09481918__mmc4-332-18-22
    Average 90 stars, based on 1 article reviews
    gentle cell dissociation reagent stem cell technologies; 07174 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Gentle:

    Article Title: Xeno-free generation of human induced pluripotent stem cells from donor-matched fibroblasts isolated from dermal and oral tissues.
    Article Snippet: Each iPS colony was cultured individually in StemFlex media (StemFlex Medium, Gibco) and considered to be a biological replica. .. Gentle Cell Dissociation Reagent (Stem Cell Technologies, Vancouver, Canada) was used for cell detachment for passaging. ..

    Article Title: Generation and genetic repair of 2 iPSC clones from a patient bearing a heterozygous c.1120del18 mutation in the ACVRL1 gene leading to Hereditary Hemorrhagic Telangiectasia (HHT) type 2.
    Article Snippet: .. Single cells were prepared with Gentle Cell Dissociation Reagent (Stem Cell Technologies) and fixed and permeabilized using the FIX & PERM Cell Fixation & Permeabilization Kit (Invitrogen) according to manufacturer's protocol. .. Cells were stained with the directly conjugated antibodies against SSEA4 and NANOG and subsequently measured using the MACSQuant VYB (Miltenyi Biotech).

    Article Title: Abortive infection of bat fibroblasts with SARS-CoV-2
    Article Snippet: .. In brief, biPSCs were cultured in DMEM (Gibco, 11330-057) supplemented with 20% knockout serum replacement (Gibco, 10828-028), 100 mM2-mercaptoethanol, 100 ng/mL FGF2 (R&D Systems, 233-FB), 10^4 U/mL Leukemia inhibitory factor (Millipore, ESG1107), 100 ng/mL SCF (R&D Systems, PHC2111), and 20 nM Forskolin (Sigma, F6886). biPSCs were initially cultured on mitomycin C-treated mouse embryonic fibroblasts and passaged with Gentle Cell Dissociation Reagent (GCDR, Stem Cell Technologies, 07174) and later maintained feeder-free on Gelatin (G2500, Sigma) for several passages before infection. .. Human lung Fibroblast cells (IMR-90, CCL-186) and Calu-3 (HTB-55) were cultured in Eagle's Minimum Essential Medium (EMEM, Sigma) supplemented with 10% fetal bovine serum, 100 U penicillin/mL, and 100 μg streptomycin/mL.

    Article Title: Spatio-temporal dynamics enhance cellular diversity, neuronal function and further maturation of human cerebral organoids.
    Article Snippet: .. When iPSCs attained a confluence of 70–80%, the cells were suspended as single cells with gentle cell dissociation reagent (Stem Cell Technologies), resuspended in hESC media [DMEM/F12 medium (Gibco) supplemented with 20% KOSR (Gibco), 3% hESCqualified FBS (Gibco), 1% Glutamax (Sigma-Aldrich), 1% NEAA (Sigma-Aldrich), 1% Penicillin/Streptomycin (Sigma-Aldrich), 0.007% BME (Sigma-Aldrich)] with 4 μg/mL bFGF (Peprotech) and 50 μM Y-27632 (Tocris) and cultured to obtain EBs and ectoderm differentiation at 9000 live cells/150 μL in a low attachment 96-well U-bottom plate (Corning) at 37 °C and 5% CO2. ..

    Article Title: Crisponi syndrome/cold-induced sweating syndrome type 2: Reprogramming of CS/CISS2 individual derived fibroblasts into three clones of one iPSC line.
    Article Snippet: 24 h later, medium was aspirated and substituted by iPSC media which then was changed three times a week. iPSC colonies with characteristic iPSC morphology were transferred to matrigel (Corning)-coated plates and cultured in mTeSR Plus medium (Stem Cell Technologies). .. Gentle cell dissociation reagent (Stem Cell Technologies) was used for the collection of CLCF1 mutated iPSCs. .. Afterwards cells were plated for trilineage differentiation according to the STEMdiffTM-Trilineage Differentiation Kit instructions (Stem Cell Technologies).

    Article Title: Interferon regulatory factor 1‐triggered free ubiquitin protects the intestines against radiation‐induced injury via CXCR4/FGF2 signaling
    Article Snippet: .. In brief, after gently washing with cold PBS, the intestines are cut into 1–2 mm long pieces and incubated with Gentle Cell Dissociation Reagent (Stem Cell Technologies, Vancouver, BC, Canada) at room temperature (15°C–25°C) for 15 min on a rocking. .. The suspension was filtered through a 70 μm cell strainer (BD Biosciences, San Diego, CA) to remove the residual villous material.

    Article Title: DNA sensor-associated type I interferon signaling is increased in ulcerative colitis and induces JAK-dependent inflammatory cell death in colonic organoids.
    Article Snippet: DNA sensor pathways can initiate inflammasome, cell death, and type I interferon (IFN) signaling in immune-mediated inflammatory diseases (IMIDs), including type I interferonopathies.. We investigated the involvement of these pathways in the pathogenesis of ulcerative colitis (UC) by analyzing the expression of DNA sensor, inflammasome, and type I IFN biomarker genes in colonic mucosal biopsy tissue from control (n = 31), inactive UC (n = 31), active UC (n = 33), and a UC single-cell RNA-Seq dataset.. The effects of type I IFN (IFN-b), IFN-c, and TNF-a on gene expression, cytokine production, and cell death were investigated in human colonic organoids.

    Article Title: Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture.
    Article Snippet: .. For DE differentiation in static suspension (3D) culture, HES3 or hCBiPSC2 were dissociated into single cells either using 0.5 μM EDTA (in PBS) or gentle cell dissociation reagent (Stem Cell Technologies). .. A defined cell number was inoculated in Costar® Ultra-Low Attachment 6-well plates (Corning) using mTeSR1 or iPS-Brew XF supplemented with 10 μM Y-27632.

    Passaging:

    Article Title: Xeno-free generation of human induced pluripotent stem cells from donor-matched fibroblasts isolated from dermal and oral tissues.
    Article Snippet: Each iPS colony was cultured individually in StemFlex media (StemFlex Medium, Gibco) and considered to be a biological replica. .. Gentle Cell Dissociation Reagent (Stem Cell Technologies, Vancouver, Canada) was used for cell detachment for passaging. ..

    Cell Culture:

    Article Title: Abortive infection of bat fibroblasts with SARS-CoV-2
    Article Snippet: .. In brief, biPSCs were cultured in DMEM (Gibco, 11330-057) supplemented with 20% knockout serum replacement (Gibco, 10828-028), 100 mM2-mercaptoethanol, 100 ng/mL FGF2 (R&D Systems, 233-FB), 10^4 U/mL Leukemia inhibitory factor (Millipore, ESG1107), 100 ng/mL SCF (R&D Systems, PHC2111), and 20 nM Forskolin (Sigma, F6886). biPSCs were initially cultured on mitomycin C-treated mouse embryonic fibroblasts and passaged with Gentle Cell Dissociation Reagent (GCDR, Stem Cell Technologies, 07174) and later maintained feeder-free on Gelatin (G2500, Sigma) for several passages before infection. .. Human lung Fibroblast cells (IMR-90, CCL-186) and Calu-3 (HTB-55) were cultured in Eagle's Minimum Essential Medium (EMEM, Sigma) supplemented with 10% fetal bovine serum, 100 U penicillin/mL, and 100 μg streptomycin/mL.

    Article Title: Spatio-temporal dynamics enhance cellular diversity, neuronal function and further maturation of human cerebral organoids.
    Article Snippet: .. When iPSCs attained a confluence of 70–80%, the cells were suspended as single cells with gentle cell dissociation reagent (Stem Cell Technologies), resuspended in hESC media [DMEM/F12 medium (Gibco) supplemented with 20% KOSR (Gibco), 3% hESCqualified FBS (Gibco), 1% Glutamax (Sigma-Aldrich), 1% NEAA (Sigma-Aldrich), 1% Penicillin/Streptomycin (Sigma-Aldrich), 0.007% BME (Sigma-Aldrich)] with 4 μg/mL bFGF (Peprotech) and 50 μM Y-27632 (Tocris) and cultured to obtain EBs and ectoderm differentiation at 9000 live cells/150 μL in a low attachment 96-well U-bottom plate (Corning) at 37 °C and 5% CO2. ..

    Knock-Out:

    Article Title: Abortive infection of bat fibroblasts with SARS-CoV-2
    Article Snippet: .. In brief, biPSCs were cultured in DMEM (Gibco, 11330-057) supplemented with 20% knockout serum replacement (Gibco, 10828-028), 100 mM2-mercaptoethanol, 100 ng/mL FGF2 (R&D Systems, 233-FB), 10^4 U/mL Leukemia inhibitory factor (Millipore, ESG1107), 100 ng/mL SCF (R&D Systems, PHC2111), and 20 nM Forskolin (Sigma, F6886). biPSCs were initially cultured on mitomycin C-treated mouse embryonic fibroblasts and passaged with Gentle Cell Dissociation Reagent (GCDR, Stem Cell Technologies, 07174) and later maintained feeder-free on Gelatin (G2500, Sigma) for several passages before infection. .. Human lung Fibroblast cells (IMR-90, CCL-186) and Calu-3 (HTB-55) were cultured in Eagle's Minimum Essential Medium (EMEM, Sigma) supplemented with 10% fetal bovine serum, 100 U penicillin/mL, and 100 μg streptomycin/mL.

    Infection:

    Article Title: Abortive infection of bat fibroblasts with SARS-CoV-2
    Article Snippet: .. In brief, biPSCs were cultured in DMEM (Gibco, 11330-057) supplemented with 20% knockout serum replacement (Gibco, 10828-028), 100 mM2-mercaptoethanol, 100 ng/mL FGF2 (R&D Systems, 233-FB), 10^4 U/mL Leukemia inhibitory factor (Millipore, ESG1107), 100 ng/mL SCF (R&D Systems, PHC2111), and 20 nM Forskolin (Sigma, F6886). biPSCs were initially cultured on mitomycin C-treated mouse embryonic fibroblasts and passaged with Gentle Cell Dissociation Reagent (GCDR, Stem Cell Technologies, 07174) and later maintained feeder-free on Gelatin (G2500, Sigma) for several passages before infection. .. Human lung Fibroblast cells (IMR-90, CCL-186) and Calu-3 (HTB-55) were cultured in Eagle's Minimum Essential Medium (EMEM, Sigma) supplemented with 10% fetal bovine serum, 100 U penicillin/mL, and 100 μg streptomycin/mL.

    Incubation:

    Article Title: Interferon regulatory factor 1‐triggered free ubiquitin protects the intestines against radiation‐induced injury via CXCR4/FGF2 signaling
    Article Snippet: .. In brief, after gently washing with cold PBS, the intestines are cut into 1–2 mm long pieces and incubated with Gentle Cell Dissociation Reagent (Stem Cell Technologies, Vancouver, BC, Canada) at room temperature (15°C–25°C) for 15 min on a rocking. .. The suspension was filtered through a 70 μm cell strainer (BD Biosciences, San Diego, CA) to remove the residual villous material.

    Suspension:

    Article Title: Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture.
    Article Snippet: .. For DE differentiation in static suspension (3D) culture, HES3 or hCBiPSC2 were dissociated into single cells either using 0.5 μM EDTA (in PBS) or gentle cell dissociation reagent (Stem Cell Technologies). .. A defined cell number was inoculated in Costar® Ultra-Low Attachment 6-well plates (Corning) using mTeSR1 or iPS-Brew XF supplemented with 10 μM Y-27632.



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